Development of 16 Microsatellite Markers for Prince’s Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
نویسندگان
چکیده
The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
منابع مشابه
Dust seed production and dispersal in Swedish Pyroleae species
Dust seeds are the smallest seeds in angiosperms weighing just about a few micrograms. Th ese seeds are characteristic of most orchids, and several studies have been performed on seed features, fecundity and dispersal of orchid dust seeds. In this study we examine seed features, seed production and seed dispersal in another plant group with dust seeds, the Pyroleae (Monotropoideae, Ericaceae), ...
متن کاملIsotopic evidence of full and partial myco-heterotrophy in the plant tribe Pyroleae (Ericaceae).
Botanists and mycologists have long debated the potential for full myco-heterotrophy in the achlorophyllous Pyrola aphylla (Ericaceae). Here we address the ecophysiology of this putative myco-heterotroph and two other closely related green species in the tribe Pyroleae (Pyrola picta and Chimaphila umbellata). The stable isotopes of carbon and nitrogen (delta13C and delta15N) were analysed from ...
متن کاملGibberellic Acid Induces Asymbiotic Germination of the Obligate Mycoheterotroph Pterospora andromedea (Ericaceae)
Studies of the Monotropoideae (monotropes; Ericaceae), a monophyletic group of nonphotosynthetic, mycoheterotrophic, and often rare or endangered plants, have been limited by the inability to propagate them. Monotropes associate with specific fungal hosts, and the only previously known method of seed germination was induction by host fungi or closely related fungi. In order to overcome very low...
متن کاملDevelopment and characterization of 23 microsatellite loci for Rhododendron ovatum (Ericaceae)1
PREMISE OF THE STUDY To estimate the genetic variation of Rhododendron ovatum (Ericaceae), a monoecious evergreen shrub, 23 microsatellite markers were identified from its nuclear genome. METHODS AND RESULTS We developed 16 polymorphic and seven monomorphic microsatellite primers using the biotin-streptavidin capture method. The 16 polymorphic loci were investigated further using 89 individua...
متن کاملDevelopment of microsatellite markers for the clonal shrub Orixa japonica (Rutaceae) using 454 sequencing1
PREMISE OF THE STUDY Microsatellite markers were developed for a dioecious shrub, Orixa japonica (Rutaceae). Because O. japonica vigorously propagates by vegetative growth, microsatellite markers can be used to identify clonal relationships among its ramets. METHODS AND RESULTS Sixteen polymorphic microsatellite markers were identified by 454 next-generation sequencing. The number of alleles ...
متن کامل